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KMID : 1103720080580040435
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Journal of the Korean Society of Radiology
2008 Volume.58 No. 4 p.435 ~ p.442
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The Impact of the Amount of Intracellular SPIO on MR Signal Intensity during In Vivo Tracking of Macrophage Homing
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Kim Dae-Yoon
Lee Jin-Seong
Kang Ju-Hee
Son Jin-Young
Kim Sang-Tae
Woo Chul-Woong
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Abstract
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Purpose: To determine whether the amount of intracellular superparamagnetic iron oxide (SPIO) in macrophages influences MR signal intensity during in vivo celluar tracking.
Materials and Methods: Peritoneal macrophages harvested from thioglycolate-treated mice were labeled with SPIO using concentrations of 112, 56, and 28 μgFe/ml, and different incubation times of 3h, 6h, 12h, 24h and 48 h, respectively. The iron concentration was quantified with the use of absorption spectrophotometry. Each group of macrophages labeled with different concentrations of SPIO was intravenously injected into 18 mice, after inoculation with S. aureus to the thigh. The relative signal intensity (SI) of the abscess wall (SI of the abscess wall/SI of muscle) was measured on MR and was analyzed by the use of the Kruskal-Wallis test.
Results: A higher concentration of SPIO in the labeling solution and a longer incubation time resulted in a higher concentration of SPIO in the macrophages. The relative SI of the abscess wall (0.63 for 112 μgFe/mL; 0.67 for 56 μgFe/ml; 0.89 for 28 μgFe/mL) significantly decreased with an increase of SPIO concentration (κ2 = 10.53, p < 0.005).
Conclusion: The amount of intracellular SPIO influences the MR signal intensity by the susceptibility effect, and it is recommended to use sufficient iron-oxide label as long as it does not affect cellular function and viability.
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KEYWORD
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Superparamagnetic iron oxide, Macrophage, Magnetic resonance(MR), Mouse
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